Table I.
Comparison of hu4D5, bD1 and bD1 variants as purified IgG1*
| Clone | Expression level (relative to bD1) | Second transition Tm (°C) | ELISA EC50 (nM) | Effective Kd (nM) [R2] |
|---|---|---|---|---|
| hu4D5 | 1.1 | 84.6 ± 0.4 | 0.08 ± 0.01 | 0.17 ± 0.06 [0.89] |
| bD1 | 1.0 | 85.1 ± 0.1 | 5 ± 2 | 90 ± 60 [0.89] |
| R2D | 0.9 | 84.8 ± 0.1 | 0.41 ± 0.08 | 3 ± 2 [0.88] |
| R2H | 0.9 | 86.0 ± 0.1 | 0.34 ± 0.04 | ND |
| R2J | 0.8 | 86.2 ± 0.1 | 0.10 ± 0.02 | 0.22 ± 0.07 [0.91] |
| R2S | 0.9 | 86.2 ± 0.1 | 0.11 ± 0.01 | 0.22 ± 0.06 [0.92] |
| R2J-S | nd | 84.7 ± 0.1 | 0.11 ± 0.01 | ND |
| R2J-Y | nd | 87.4 ± 0.1 | 0.12 ± 0.02 | ND |
ND: no data.
*Errors are standard deviation for Tm, range of duplicates for EC50 and standard error for Kd.