Skip to main content
. 2018 Mar 16;31(3):91–101. doi: 10.1093/protein/gzy004

Table I.

Comparison of hu4D5, bD1 and bD1 variants as purified IgG1*

Clone Expression level (relative to bD1) Second transition Tm (°C) ELISA EC50 (nM) Effective Kd (nM) [R2]
hu4D5 1.1 84.6 ± 0.4 0.08 ± 0.01 0.17 ± 0.06 [0.89]
bD1 1.0 85.1 ± 0.1 5 ± 2 90 ± 60 [0.89]
R2D 0.9 84.8 ± 0.1 0.41 ± 0.08 3 ± 2 [0.88]
R2H 0.9 86.0 ± 0.1 0.34 ± 0.04 ND
R2J 0.8 86.2 ± 0.1 0.10 ± 0.02 0.22 ± 0.07 [0.91]
R2S 0.9 86.2 ± 0.1 0.11 ± 0.01 0.22 ± 0.06 [0.92]
R2J-S nd 84.7 ± 0.1 0.11 ± 0.01 ND
R2J-Y nd 87.4 ± 0.1 0.12 ± 0.02 ND

ND: no data.

*Errors are standard deviation for Tm, range of duplicates for EC50 and standard error for Kd.