Fig 5. Schematic representation of peptide fragments used in bacterial two-hybrid assays and the growth results of E. coli expressing FcpA and the FlaA2 derivatives (A) and the FlaB1 derivatives (B) in the selective medium supplemented with 3-AT and streptomycin.

No E. coli transformants were obtained when transformed with the plasmids encoding FlaA2 fragments A2-2 (1–154), A2-3 (1–223), or A2-5 (85–223) and with the plasmid encoding FlaB1 fragment B1-1 (1–144).