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HPLC elution profiles of in vitro 14C-IAA and/or 3H-IAA catabolism. A, 5-3H-IAA plus Mn2+ plus p-coumaric acid. B, 5-3H-IAA plus Mn2+ plus p-coumaric acid plus AAO. C, 1-14C-IAA plus Mn2+ plus p-coumaric acid. D, 1-14C-IAA plus AAO plus Mn2+ plus p-coumaric acid. Peak 1, Oxindole-3-methanol, the primary in vitro catabolic product; peak 2, IAA; peak 3, 3-methyleneoxindole, a non-biological breakdown product of peak 1.
