Table 2. Sequencing primers for four NSHL associated loci.
Genes | Mutations | Primer names | Primer sequence(5'-3') | Bases(bp) | Purification |
---|---|---|---|---|---|
GJB2 | c.235delC | GJB2-246-F1 | AGAGTTGGTGTTTGCTCAGG | 20 | PAGE |
GJB2-1011-R1 | TTCAGTGACATTCAGCAGGA | 20 | PAGE | ||
SLC26A4 | c.919-2A>G | SLC26A4-253-F1 | GATTTCACTGCTGGATTGCT | 20 | PAGE |
SLC26A4-756-R1 | GCATATACGGGCTGCTTTTA | 20 | PAGE | ||
MTRNR1 | m.1555A>G/m.1494C>T | MTRNR1-192-F1 | TAATCGATAAACCCCGATCA | 20 | PAGE |
MTRNR1-761-R1 | TATCTATTGCGCCAGGTTTC | 20 | PAGE |