. 2018 Mar 26;12(3):e0006330. doi: 10.1371/journal.pntd.0006330

Table 3. Summary table of total antibody ICT.

Assay	Study	Year	Location of sample collection	Sample collection timing	Reference test assay type	Reference assay positivity cut-off	Sensitivity (%)/ specificity (%)
CareStart, AccessBio	Blacksell et al^a [28]	2010	Laos and Thailand	Acute phase	IFA and/or PCR and/or culture	IFA: 4-fold rise in paired serum samples	97.6/71.4
CareStart, AccessBio	Blacksell et al^a [19]	2012	Thailand	Acute (median 5 days of fever, IQR 3 to 7 days) and convalescent phase	STIC	IFA: an admission IgM titre of 1:12,800, and/or a 4-fold rising IgM titre in paired serum samples	60.5/67.9
ImmuneMed	Kim et al^a [39]	2013	Korea	Unclear	Unclear	Unclear	99.1/98.9
	Kim et al^b [40]	2016	Korea^d	Unclear	IFA IgM	1:10	98.6/98.2
			Korea^d	Unclear	IFA IgG	1:40	97.1/97.7
			Korea^e	Unclear	IFA IgM	1:10	98.6/97.6
			Korea^e	Unclear	IFA IgG	1:40	97.1/97.0
			Sri Lanka	Unclear	IFA	Unclear	92.1/96.1
			India	Unclear	IFA IgM	1:10	Sensitivity 86.0%
			India	Unclear	IFA IgG	1:40	Sensitivity 92.0%
	Stephen et al^b [51]	2016	India	Acute and convalescent phase	IFA	1:80	77.3/86.4
InBios	Zhang et al^b [26]	2011	China	Acute phase: 2 to 10 days after onset of illness	IgM and IgG antibodies detection (unclear) and PCR	Unclear	Sensitivity 90.9%
InBios	Silpasakorn et al^c [48]	2012	Thailand	Acute and convalescent phase	PCR & IFA	IFA: IgG 1:800 or a 4-fold increase	66.7%/98.4
PanBio	Coleman et al^b [36]	2002	Thailand	Acute phase	IIP	1:400	90.4/95.8
Panbio prototype	Wilkinson et al^b [54]	2003	Thailand, Australia	Unclear	Unclear	Unclear	96.1/93.0
SD Bioline Tsutsugamushi	Silpasakorn et al^c [49]	2012	Thailand	Acute phase: median 6 days of fever (range 1–47 days); median interval between obtaining admission and convalescence phase: 13 days (range 3–32 days)	PCR & IFA	IFA: IgM or IgG IFA assay titre > 1:400 or a 4-fold increase	66.7/98.4
	Lee et al^c [27]	2014	Korea	Unclear	IFA	4-fold rise or single titre ≥ 1:160	Sensitivity 72.6%
	Stephen et al^c [50]	2015	India	Acute and convalescence phase	ELISA IgM	Unclear	91.7/90.5
	Stephen et al^c [50]	2015	India	Acute and convalescence phase	ELISA IgG	Unclear	85.7/100.0
	Watthanaworawit et al^c [29]	2015	Thailand	Median fever = 2 days (IQR: 2–3 days), and the median interval between obtaining initial acute-phase specimens and convalescent specimens was 14 days (range: 11–30 days).	PCR & IFA	≥ 4-fold increase in IFA IgM titre, 1:25,600	Acute samples: 20.9/74.4
	Watthanaworawit et al^c [29]	2015	Thailand		PCR & IFA	≥ 4-fold increase in IFA IgM titre, 1:25,600	Paired samples: 76.7/76.7
In-house tests	Ching et al^a [24]	2001	Unclear	Unclear	IFA	>1:40	IgG: 86.0/97.0
	Ching et al^a [24]	2001	Unclear	Unclear	IFA	>1:40	IgG, IgM: 89.0/97.0
	Cao et al^a [25]	2007	China	Unclear	Unclear	Unclear	IgG: 94.6/98.9
	Cao et al^a [25]	2007	China	Unclear	Unclear	Unclear	IgG, IgM: 98.2/98.1

^aTotal antibody: IgG & IgM

^bIgG

^cIgG, IgM, IgA

^dHealthy control sera

^eDiseases non-scrub typhus control sera