Table 3. Mutation combinations with synthetic rescue features.

Class	Starting Strain	Deleterious Mutation(s)	Rescue Mutation(s) a	Growth Media
Forward synthetic rescues (S1C Fig)	BW25113	ΔdapF	4,174,048 A > G; 928,451 C > A	LB
		ΔdapF	4,173,309 G > T; 928,451 C > A	LB
		Δzwf	4,178,209 C > A; 1,301,660 IS5(+)	LB
Reverse synthetic rescues (S1F Fig) b	ΔdapF-sup2	4,173,309 T > G; 928,451 A > C	Restore dapF	M9G
	Δdgk-sup1	4,178,500 T > C	Restore dgk	M9G
	Δdgk-sup2	4,172,748 T > C	Restore dgk	M9G
	ΔentC-sup3	2,374,180 A > G; 4,178,500 T > C	Restore entC	M9G

^a All rescue mutations are neutral or deleterious in the starting strain background.

^b Mutations are applied in the reverse order from which they occurred in our experiments.