Figure 8.

(A) Histopathologic assessment of myocardial damage; (B) H₂S level in Sham, DM-8w, Curcumin, Curcumin/P, Curcumin+PAG, Curcumin/P+PAG, Curcumin+ CaRS+PAG, and Curcumin/P+CaRS+PAG groups; (C) CSE expression in Sham, DM-8w, Curcumin, Curcumin/P, Curcumin+PAG, Curcumin/P+PAG, Curcumin+CaRS+PAG, and Curcumin/P+CaRS+PAG groups.

Notes: (A) Light microscope images (×100) in Sham group (a), DM-8w group (b), Curcumin group (c), Curcumin/P group (d), Curcumin+PAG group (e), Curcumin/P+PAG group (f), Curcumin+CaRS+PAG group (g), and Curcumin/P+CaRS+PAG group (h). The quantitative analysis of histopathologic assessment is showed in “i”. The histopathologic damage in DM-8w group is higher than that in Sham group (p<0.01), and the histopathologic damage in Curcumin group, Curcumin+PAG group, and Curcumin+CaRS+PAG group is lower than that in Sham group (p<0.01); the histopathologic damage in Curcumin/P group, Curcumin/P+PAG group, and Curcumin/P+CaRS+PAG group is significantly lower than that in Sham group (p<0.01). (B) The blood of Sham, DM-8w, Curcumin, Curcumin/P, Curcumin+PAG, Curcumin/P+PAG, Curcumin+CaRS+PAG, and Curcumin/ P+CaRS+PAG groups were collected and the levels of H₂S were measured. Results are expressed as mean ± SD. (C) The cardiac tissues of Sham, DM-8w, Curcumin, Curcumin/P, Curcumin+PAG, Curcumin/P+PAG, Curcumin+CaRS+PAG, and Curcumin/P+CaRS+PAG groups and the expression of CSE were measured. Results are expressed as mean ± SD. A significant decrease from Sham group is denoted by *p<0.01, a significant increase from DM-8w group is denoted by **p<0.01, and a significant increase from DM-8w group is denoted by ***p<0.01.

Abbreviations: CaSR, calcium-sensing receptor; DM, diabetes mellitus; CSE, cystathionine-γ-lyase; CaRS, calcium-sensing receptor stimulator; PAG, DL-propargylglycine; CaM, calmodulin; P, poly(gamma-benzyl l-glutamate)-poly(ethylene glycol)-poly(gamma-benzyl l-glutamate); SD, standard deviation.