Table 1.
Oocytes | No. of oocytes cultured | No. (%) of oocytes matured | Diameter of cytoplasm (μm) | Inner diameter of zona pellucida (μm) | Thickness of zona pellucida (μm) | Size of perivitelline space (μm) |
---|---|---|---|---|---|---|
Immediately after collection from antral follicles | 30 | – | 125.4 ± 1.01A,a | 125.6 ± 0.96 A | 13.08 ± 0.33 A | 0.12 ± 0.07c |
Ovulated | 30 | 30 (100.0) A | 99.54 ± 0.60b | 122.6 ± 0.73b | 14.20 ± 0.28 A | 11.51 ± 0.34 A |
Cultured | 41 | 28 (68.3)b | 92.61 ± 0.88c | 112.0 ± 0.89c | 13.48 ± 0.31 A | 9.68 ± 0.44b |
MU‐treated | 42 | 28 (66.7) A | 98.66 ± 0.57 A | 113.1 ± 0.78b | 16.09 ± 0.31 A | 7.13 ± 0.39b |
Control | 47 | 30 (63.8) A | 98.13 ± 0.77 A | 115.6 ± 0.78 A | 16.15 ± 0.36 A | 8.75 ± 0.45 A |
AMean ± SE
The oocytes with cumulus cells were cultured for 14 h in BO medium with 0.25 mm MU (MU‐treated) or in BO medium without MU (control)
Values with different superscripts in the same column in each group of experiments are significantly different (p < 0.05)