Figure 1.
Testosterone‐induced primordial follicle activation in porcine ovarian strips. The ovarian strips were fixed in paraformaldehyde and stained with hematoxylin and eosin. The ovarian strips before culture (a) contained mainly healthy primordial follicles. 10−6 m testosterone treatment for 7 days yielded intermediate follicles (arrow in c) and primary follicles (double arrow in d). Ovarian strips treated with estradiol‐17β (e), cyproterone acetate + testosterone (f), and control treated with vehicle (b), in which the primordial follicles were intact, had not started to develop after 7 days. Scale bar represents 40 µm