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Developmental rate of zona‐free eggs to two‐cell embryos; comparison between collagenase and acidified Tyrode's solution treatment
| Method of zona removal | Number of eggs | |
|---|---|---|
| Total | Two‐cell embryo rate (%) | |
| Collagenase (mg/ml) | ||
| 1.0 | 294 | 235 (79.9)a |
| 0.1 | 112 | 85 (76)a |
| Acidified Tyrode's solution | 108 | 65 (60)b |
Values with the same superscript are not significantly different. a–b: P < 0.05
