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Comparison of zona removal rate between collagenase and acidified Tyrode's solution treatment; effect of post‐ovulatory aging
| Method of zona removal | Status of post‐ovulatory eggs | Number of eggs | |
|---|---|---|---|
| Total | Zona removal rate (%) | ||
| Collagenase (0.1 mg/ml) | Young | 227 | 182 (80.2)a |
| Aged | 175 | 30 (17)b | |
| Acidified Tyrode's solution | Young | 174 | 165 (94.8)c |
| Aged | 28 | 26 (93)c | |
Values with same superscript are not significantly different. a–b, a–c, b–c: P < 0.01
