Table 1. Total cell count in RGCL and optical dissector parameters.
Groups name | Control n=6 | IR n=6 | Bevacizumab n=6 |
---|---|---|---|
Mean Total Retinal Ggl Cell Count ± SEM |
203.712±27.749 |
131.133±16.025 |
173.289±5.336 |
Dissector Particle Number |
446 |
319 |
393 |
Section Thickness (µm) |
20,5 |
19,5 |
19,7 |
Number of Sampled Sections |
25,2 |
23 |
24 |
CE |
0,05 |
0,05 |
0,05 |
CV | 0,09 | 0,14 | 0,09 |
Mean total ganglion cell count ±SEM, mean dissector number, section thickness, number of sampled sections, coefficient of error (CE), and coefficient of variation (CV) of stereological analysis between subjected to ischemia/reperfusion and treatment groups in the rodents. The stastical difference between all the groups is significant ; between C and IR p<0.0001, C and BEV p<0.01 and IR and BEV p<0.01. The neurodegenerative effects of IR and the neuroprotective effects of BEV were examined by inducing 45 min of an ischemic insult and following a reperfusion of 48 h. There were 203.712±27.749 cells in the RGCL in the control group and 131.133±16.025in the IR group, showing a 36% decrease in living cells as a strong sign of neurodegeneration (p<0.0001). When we compared the cell numbers in the BEV group 173.289±5.336to the IR group 131.133±16.025we clearly saw there was a higher number of cells in the BEV group, and the difference was statistically significant (p<0.01). This is a strong clue that BEV has a neuroprotective effect in the event of ischemia.