Diagrammatic representation of the segments of the nephron modified from Nguyen et al., 2012 [42] and choice of promoters for segment-specific gene expression and AAV vector promoter constructs. PT = proximal tubule, DL = descending limb of the loop of Henle, AL = ascending limb of the loop of Henle, TALH = thick ascending limb of the loop of Henle, MD = macula densa, DCT = distal convoluted tubule, CNT =connecting tubule, CD = collecting duct, CMV = cytomegalovirus, eGFP = enhanced green fluorescent protein, SV-pA = Simian virus 40 polyadenylation signal sequence, ITR = inverted terminal repeat sequence, KSPC = kidney-specific cadherin, SGLT2 = sodium-glucose cotransporter 2, NKCC2 = sodium potassium 2-chloride cotransporter, ECAD = e cadherin.