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. Author manuscript; available in PMC: 2019 Mar 5.
Published in final edited form as: Curr Biol. 2018 Feb 22;28(5):779–787.e3. doi: 10.1016/j.cub.2018.01.060

Figure 2. Ilp6 in the fat body is necessary and sufficient for regulation of the starvation-induced reduction in Tp.

Figure 2

Comparison of Tp between fed (F: fed, white box) and starved overnight (S: starved O/N, gray box) conditions in the groups of flies indicated below.

(A) yw (control), ilp6 loss-of-function (LOF) (ilp6 LOF) and ilp6 LOF rescue (ilp6 LOF; Cg-Gal4/uas-ilp6) flies, in which ilp6 is expressed in the fat body using Cg-Gal4 in the ilp6 LOF mutant background. Whiskers show the minimum-maximum data range of Tp, and boxes show the 25–75th percentile data range of Tp. A horizontal line and a cross in each box represent the median and mean, respectively. Italicized numbers indicate the number of trials. A t-test was performed to compare Tp under fed and starved conditions in each genotype. The behavioral experiments performed at ZT 1–3, 4–6, 7–9 and 10–12 are shown in Figure S2B.

(B) yw (control), ilp6 null and ilp6 null rescue (ilp6 null; Cg-Gal4/uas-ilp6) flies, in which ilp6 is expressed in the fat body using Cg-Gal4 in the ilp6 null mutant background. In the fed condition, the Tp of both ilp6 LOF and ilp6 null flies was lower than that of yw flies, however, it did not differ from that of the rescued flies (Table S1).

(C) RNAi knockdown of ilp6 in the fat body: CgG4/+ (Cg-Gal4/+), ilp6-RNAi/+ (uas-ilp6-RNAi/+) and CgG4> ilp6-RNAi (Cg-Gal4/uas-ilp6-RNAi) flies.

(D) RNAi knockdown of ilp6 in the fat body: 3.1Lsp2G4/+ (3.1Lsp2-Gal4/+), ilp6-RNAi/+ and 3.1Lsp2G4> ilp6-RNAi (uas-ilp6-RNAi/+; 3.1Lsp2-Gal4/+) flies.

The plotting pattern and statistical analysis are the same as in Figure 2A. *p<0.05. **p<0.01. NS indicates no significance. See also Figure S2, S3, Table S1 and S2.