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. 2018 Jan 5;32(4):1916–1932. doi: 10.1096/fj.201700826R

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This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (http://creativecommons.org/licenses/by-nc/4.0/) which permits noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Neuronal NF-κB inhibition leads to up-regulation of proapoptotic factors after CHI. A–G) Expression of genes regulating apoptosis 3 d after TBI. Proapoptotic, but not antiapoptotic, factors were significantly elevated in the cortical impact area of IKK2-DN^Camk2a mice, but not in IKK2-CA^Camk2a mice vs. control littermates. qPCR analysis of the proapoptotic genes Bax (A), Bad (B), and Bak (C); the antiapoptotic genes Bcl2 (D) and Bcl_XL (E); and the stress response genes in the p53 pathway Atf3 (F) and p21 (G). Expression levels measured by qPCR are presented relative to Hprt. Means ± sem (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 [not significant (ns), at 3 d TBI between the indicated groups, by 1-way-ANOVA followed by Bonferroni’s post hoc test]. H) Expression of proapoptotic proteins after trauma. Representative immunoblot analysis and quantification of BAX and BAD protein levels in brain lysates of 12-wk-old control and IKK2-DN^Camk2a mice. TUBB is used as loading control. Means ± sem (n = 3). *P < 0.05, ***P < 0.001 (ns, 3 d after CHI, between the indicated groups, by 1-way-ANOVA followed by Bonferroni’s post hoc test).

Figure 5. — Neuronal NF-κB inhibition leads to up-regulation of proapoptotic factors after CHI. A–G) Expression of genes regulating apoptosis 3 d after TBI. Proapoptotic, but not antiapoptotic, factors were significantly elevated in the cortical impact area of IKK2-DN^Camk2a mice, but not in IKK2-CA^Camk2a mice vs. control littermates. qPCR analysis of the proapoptotic genes Bax (A), Bad (B), and Bak (C); the antiapoptotic genes Bcl2 (D) and Bcl_XL (E); and the stress response genes in the p53 pathway Atf3 (F) and p21 (G). Expression levels measured by qPCR are presented relative to Hprt. Means ± sem (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 [not significant (ns), at 3 d TBI between the indicated groups, by 1-way-ANOVA followed by Bonferroni’s post hoc test]. H) Expression of proapoptotic proteins after trauma. Representative immunoblot analysis and quantification of BAX and BAD protein levels in brain lysates of 12-wk-old control and IKK2-DN^Camk2a mice. TUBB is used as loading control. Means ± sem (n = 3). *P < 0.05, ***P < 0.001 (ns, 3 d after CHI, between the indicated groups, by 1-way-ANOVA followed by Bonferroni’s post hoc test).