Figure 6.

Treatment with Ca²⁺ causes a decrease of [¹⁴C]et-eEF1A recovered from microsomes. Ca²⁺ (1, 10, 100, and 1,000 μm) was added to microsomes (112 μg of membrane protein) prepared as described in Figure 2. The reaction was incubated at 25°C with shaking (200 rpm) for 10 min. The reaction was stopped by adding ice-cold EGTA. An aliquot of each reaction (20 μL) was separated by 10% (w/v) SDS-PAGE. The gel was dried and exposed to a phosphor imager tritium screen for 1 week.