Table I.

Phospholipase Dα causes a decrease of [¹⁴C]et-eEF1A recovered from microsomes

Sample	[14C]et-EF1A
	dpm/mg
MES buffer	32,250 ± 10,000
Vector alone	47,375 ± 13,812
Phospholipase Dα	16,875 ± 9,870

Carrot microsomes (30 μg of protein) resuspended in 20 mm Tris-HCl (pH 6.5) were added to reaction buffer consisting of 100 mm MES (pH 6.5), 5 mm CaCl₂, 0.5 mm SDS, and 1% (v/v) ethanol. The reaction was incubated at 30°C in a shaking water bath for 30 min. Aliquots were removed from each reaction for recovery of [¹⁴C]et-eEF1A by TCA precipitation analysis. The means of four values from two experiments are reported.