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. 2018 Mar 27;9(23):16284–16296. doi: 10.18632/oncotarget.24616

Figure 4. SGE inhibits LPS-induced NO production and MAPK/NF-κB activation in Raw 264.7 cells.

Figure 4

(A) Cells were pretreated with or without the indicated concentrations of SGE or dexamethasone (10 μM) for 1 h and then stimulated with LPS (200 ng/mL). After 24 h, the levels of NO in the culture supernatants were measured. The data are representative of three independent experiments and expressed as means ± SD performed in triplicate. (B) The mRNA and protein levels of iNOS in cells treated as described in (A) were examined by RT-PCR and Western blotting, respectively. The data were expressed as means ± SD of two independent experiments. (C) Cells pretreated with or without the indicated concentrations of SGE for 1 h were stimulated with LPS (200 ng/mL) for 1 h and then subjected to Western blotting. The band intensities relative to the SGE-untreated cells were determined and represented as means ± SD from two independent experiments. #p < 0.01 vs. untreated control, *p < 0.05 and **p < 0.01 vs. SGE-untreated control.