Figure 1. TpeL_GTD glycosylates Ras proteins irrespective of nucleotide binding and hyperactive mutations in vitro.

(A) Relative initial glycosylation velocities were calculated from three independent experiments for K-Ras preloaded with GDP, GTP or GDP-AlF₃. (B) Wild type as well as G12V mutated K-, H-, and N-Ras proteins were glycosylated by TpeL_GTD. Shown are the autoradiogram (upper panel) and the Coomassie-stained gel (lower panel).