Figure 3. TpeL treatment reduces Ras-dependent hyperproliferation of melanocytes in vivo.

(A) Micrographs of zebrafish larvae with Ras-dependent hyperpigmentation at 60 hpf treated with low (1 nM) and high (30 nM) concentrations of TpeL from 12 hpf on. A reduced pigmentation in the high TpeL concentration treated fish is seen over the whole larvae. The scale bar represents 0.1 mm. (B) TpeL- or untreated zebrafish larvae were pooled to 20 embryos per condition and lysed. Subsequently, Ras_non-gluc., pMEK, pERK, tERK, and tubulin were probed by immunoblotting. A representative blot of three independent experiments is shown. (C) Statistical analysis of phosphorylated MEK and ERK following treatment with TpeL as presented in B.