Figure 4.

The distinct role of LIC2 and HC1 in 3D cell migration. A–F). Typical trajectories of 25 individual control, LIC2-, and HC1-depleted cells migrating on collagen I–coated 2D substrates and inside 3D collagen I matrices. Scale bar, 200 µm. G, H). shRNA-mediated depletion of LIC2 has an inhibitory effect on cell migration in cells migrating in collagen I matrices (H) but has no significant effect on cell migration on substrates (G). I, J). Regulation of migration for cells on 2D substrates (I) and in 3D collagen I matrices (J) by LIC2. MSDs were evaluated at a time scale of 1 h (I, J). K). Total number of mother protrusions (zeroth-generation protrusions) generated per 90 min per cell. L, M). Number of first-generation protrusions (L) and second-generation protrusions (M) generated per 90 min per cell. Insets: Number of first-generation protrusions per mother protrusion (L); number of second-generation protrusions per first-generation protrusion (M). N). Total number of protrusions generated per 90 min per cell. For all panels, cells were monitored for 16.5 h. For each condition, n = 3; at least 60 cells were probed. *P < 0.05, ***P < 0.001.