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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 12;200(8):2809–2818. doi: 10.4049/jimmunol.1700691

Fig. 3. TLR9-mediated activation of signaling kinases needs EGFR activity.

Fig. 3

(A–D) RAW264.7 cells were pre-treated with DMSO or gefitinib (Gf, 10 μM) for 1 h and were stimulated with CpG (10 μg/ml) for the indicated times in the presence of DMSO or Gf. The cell lysates were analyzed for (A) pIkBα (on Ser32) and total IkBα; (B) pERK1/2 (on Thr202/Tyr204), pJNK1/2 (phosphor- p54/p46); (C) pTBK1 (on Ser172) and total TBK1 and (D) pAKT (on Ser473) and total AKT. Actin was used as a loading control. The data are representative of at least three independent experiments.