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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Cell Microbiol. 2018 Feb 7;20(5):e12825. doi: 10.1111/cmi.12825

Figure 4. Ndk-deficient mutant strain of P. gingivalis does not inhibit Staurosporine-induced Cytochrome C release in primary GECs.

Figure 4

Uninfected and infected GECs, with either Wild-type (WT) or ndk-deficient (ΔNdk) P. gingivalis at MOI 100, were incubated with pro-apoptotic agent, Staurosporine (STP) [1μM] for 3 hours. Cells were immunostained for Cytochrome C (red) and P. gingivalis (green) and mounted with DAPI. Weaker and largely dispersed red fluorescence indicates release of Cytochrome C from the mitochondria into the cytosol of GECs. ~100 cells were examined for each condition. The assays were repeated at least in three separate experiments. 40x images were taken on Zeiss Axio Imager 1; Bar 10μm.