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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Mol Cell Neurosci. 2017 Dec 12;88:43–52. doi: 10.1016/j.mcn.2017.12.005

Figure 2. p62 is transcriptionally up-regulated in response to proteotoxic stress.

Figure 2

A. Representative Western blot analysis of p62 levels in response to MG132 treatment. Cells were treated with 10 µM MG132 in the presence or absence of 10 µg/ml cycloheximide (CHX) as indicated for 6 hours. Actin was used as the loading control. B. Quantification of p62 expression by densitometry. Cells were treated with 10 µM MG132 for 6 hours. * p<0.05; ** p<0.01, compared to their respective controls. N=9–10 for each group. Student’s t-test. C. Quantitative RT-PCR measurement of p62 mRNA. Cells were incubated with MG132 for 6 hours. ** p<0.01, Two-way ANOVA with the Tukey’s post-hoc analysis. There is a significant main effect for treatment, F(1, 12)=17.82, p=0.0012; no significant effect for cell types, F(1, 12)=1.537, p=0.2387 and no significant interaction between cell types and treatments, F(1, 12)=1.668, p=0.2208.