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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Mol Cell Neurosci. 2017 Dec 12;88:43–52. doi: 10.1016/j.mcn.2017.12.005

Figure 5. p62 puncti are associated with proteasomes in STHdhQ7 cells but not Q111 cells after MG132 treatment.

Figure 5

A–B. Representative confocal images showing that p62 puncti co-localize with the 20S proteasomal subunit in STHdhQ7 (A) but not Q111 (B) cells after 2 hours of MG132 treatment (right panels). The subcellular localization of p62 and 20S proteasomal subunit in untreated cells are also shown in the left panels. C. Manders’ co-localization coefficiency analysis for the percentage of p62 puncti that are positive for 20S proteasomal subunit. N=9 cells per group. ****p<0.0001, student’s t-test. D–E. Representative confocal images showing that p62 puncti co-localize with UBQLN2 in STHdhQ7 (D) but not Q111 (E) cells after 2 hours of MG132 treatment (right panels). The subcellular localization of p62 and UBQLN2 in untreated cells is also shown in the left panels. F. Manders’ co-localization coefficiency analysis for the percentage of p62 puncti that are positive for UBQLN2. N=10–11 cells per group. ****p<0.0001, student’s t-test.