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. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 12;200(8):2563–2570. doi: 10.4049/jimmunol.1701200

FIGURE 3.

FIGURE 3

Biotin deficiency decreases the proliferation CD4+ T cells. CFSE-labeled CD4+ T cells were cultured in biotin-deficient and control medium for 72 h. (A) Dot plots depict the dilution of CFSE dye as a measure of proliferation. (B) CD4+ T cells cultured under biotin-deficient and -sufficient conditions for 72 h were stained with 7-AAD to determine cell death. Dot plots depict the percentage of 7-AAD+ T cells in the culture. Bar graph depicts the same. Data are mean ± SE of four experiments. *p < 0.05; **p < 0.01.