Skip to main content
. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 12;200(8):2847–2859. doi: 10.4049/jimmunol.1701464

FIGURE 4.

FIGURE 4

All cloned variants of RT1-U functions as ligands for Ly49s4/-i4/-i3. (A) Amino acid sequence of the different allelic variants of RT1-U used in this study, i.e. Un (corresponds with CE16 in the n haplotype), Uav1, Ulv1, and Uc/d and RT1-Cl. (B) Testing of the response of Ly49s4/-i4/-i3 reporter cells against YB2/0 stimulator cells stably transfected with the different RT1-U alleles. As negative and positive controls are shown reporters incubated with wild-type YB2/0 cells (YB) or with Ionomycin (io.) (C) Cytolytic activity of DAR13+ IL-2 activated NK cells generated from spleens of normal PVG and F344 rats was tested against Con A lymphoblast targets from the MHC congenic strains PVG.1N (n), PVG.LV1 (lv1), and PVG (c), in the presence of blocking quantities of mAb DAR13 (+) or the isotype control mAb FLY5 (-). Results are only shown for the effector:target cell ratio of 50:1. Data are representative of at least 3 independent experiments, *p<0.01.