Skip to main content
. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 12;200(8):2847–2859. doi: 10.4049/jimmunol.1701464

FIGURE 6.

FIGURE 6

Specific adaptation of the NK cell repertoire to repeated alloimmunizations in vivo. (A) Congenitally athymic nude rats (c haplotype) were immunized once weekly for a total of four weeks i.p. with mononuclear splenocytes from PVG.1U (u) or PVG.1N (n) rats. Nude rats receiving PBS only were used as negative controls (Ctr.). Peritoneal cells were retrieved one week after the last injection and analysed by flow cytometry, gating on CD3−NKR-P1A+ NK cells. Data are representative of 4 experiments, the percentage of positive cells are given. (B) Cytolytic activity of ex vivo peritoneal cells from nude rats immunized with PVG.1U (ImmU) or PVG.1N (ImmN) or non-immunized nude controls (Ctr.) against lymphoblast target cells from PVG.1U (u), PVG.1N (n) and PVG.R23 (u-a-av1) rats. Data are representative of at least 3 independent experiments, *p<0.01.