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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 12;200(8):2847–2859. doi: 10.4049/jimmunol.1701464

FIGURE 7.

FIGURE 7

Allostimulation with an RT1-Un transfectant induces highly alloreactive Ly49s4/-i4/-s3/-i3+ NK cells in vivo. (A) PVG.1U rats were immunized once weekly for a total of four weeks i.p. with RT1-Un transfected YB2/0 cells (YB.Un), wild-type YB2/0 cells (YB2/0) or PBS only. Proportions of lymphocytes, T cells (CD3+), NK-T cells (CD3+NKR-P1+), NK cells (CD3−NKR-P1A+), DAR13+ NK cells and NKR-P1B+ NK cells were evaluated by flow cytometry, data for individual rats (n=4–13) are given for each group, *p<0.01. (B) Two-color plots showing the two dominant DAR13+ and NKR-P1B+ NK subsets in the three experimental groups. (C) Cytolytic activity of ex vivo peritoneal cells from immunized (YB.Un) versus control (Ctr.) PVG.1U rats against Con A-activated lymphoblast target cells from PVG.1N (n), PVG.R23 (u-a-av1), or syngeneic PVG.1U (u) rats. Data are representative of at least 3 independent experiments, *p<0.01 (D) qPCR of Ly49s4, -i4 and -s3 expression among DAR13+ sorted NK cells from PVG.1U rats immunized with transfected YB.Un cells compared to untransfected YB2/0 cells as control (YB Ctr.), *p<0.05.