Skip to main content
. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: Hepatology. 2018 Jan 2;67(2):560–574. doi: 10.1002/hep.29588

Figure 1. ITPR2 in hepatocytes is transcriptionally regulated by c-Jun.

Figure 1

(A) DNA sequence of the proximal region (2 kb) of the human ITPR2 promoter. Potential transcriptional binding sites for c-Jun/AP-1 are shaded in gray, and underlined nucleotides are the core sequence of AP-1. Numbered positions refer to the transcription starting sites (nucleotide, +1, arrow). (B) c-Jun inhibits ITPR2 promoter activity in a dose-dependent manner in Huh7 cells and (C) in HepG2 cells. Results are presented as fold changes relative to empty luciferase vector (pGL4) from the same dual luciferase assay. *p < 0.05, compared with 0 ng of c-Jun plasmid; Huh7 (n = 6), HepG2 (n = 3). (D) Relative mRNA expressions of ITPR2 (left panel) and c-Jun (right panel) in Huh7 cells transfected with various concentrations of c-Jun plasmid for 24 hours. c-Jun overexpression directly decreases ITPR2 mRNA expression in Huh7 cells transfected with c-Jun plasmids. *p < 0.05, compared with 0 ng of c-Jun plasmid (n = 4). (E) Representative immunoblot and (F) Relative protein expressions of ITPR2 (left panel) and c-Jun (right panel) in Huh7 cells transfected with c-Jun plasmids, measured after 48 hours. *p < 0.05, compared with 0 ng of c-Jun plasmid (n = 3).