Table 1. Association of selected microRNA ratios and 5-year cancer recurrence rates in patients with early-stage non-small cell lung cancer.
| Expression levle | Discovery | Validation | Combined | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Recurrence (N, %) |
No recurrence (N, %) |
HRa (95% CI) |
P | Recurrence (N,%) |
No recurrence (N,%) |
HRa (95% CI) |
P | Recurrence (N,%) |
No recurrence (N,%) |
HRa (95%CI) |
P | ||
| miR-18a/25 | Low | 10 (45.45) | 12 (54.55) | 1 | 8 (33.33) | 16 (66.67) | 1 | 18 (39.13) | 28 (60.87) | 1 | |||
| High | 9 (20.00) | 36 (80.00) | 0.27 (0.09-0.76) | 1.33E-02 | 11 (24.44) | 34 (75.56) | 0.28 (0.09-0.87) | 2.69E-02 | 20 (22.22) | 70 (77.78) | 0.3 (0.14-0.63) | 1.55E-03 | |
| miR-18a/93 | Low | 9 (42.86) | 12 (57.14) | 1 | 11 (42.31) | 15 (57.69) | 1 | 20 (42.55) | 27 (57.45) | 1 | |||
| High | 9 (18.75) | 39 (81.25) | 0.20 (0.06-0.66) | 8.03E-03 | 10 (21.74) | 36 (78.26) | 0.27 (0.1-0.76) | 1.33E-02 | 19 (20.21) | 75 (79.79) | 0.3 (0.15-0.61) | 9.05E-04 | |
| miR-18a/328 | Low | 10 (50.00) | 10 (50.00) | 1 | 11 (44.00) | 14 (56.00) | 1 | 21 (46.67) | 24 (53.33) | 1 | |||
| High | 9 (20.45) | 35 (79.55) | 0.17 (0.06-0.52) | 1.95E-03 | 8 (17.78) | 37 (82.22) | 0.17 (0.05-0.52) | 2.16E-03 | 17 (19.10) | 72 (80.90) | 0.18 (0.08-0.39) | 1.04E-05 | |
| miR-19b/339-3p | Low | 5 (14.29) | 30 (85.71) | 1 | 6 (17.14) | 29 (82.86) | 1 | 11 (15.71) | 59 (84.29) | 1 | |||
| High | 12 (35.29) | 22 (64.71) | 3.42 (1.10-10.65) | 3.36E-02 | 15 (40.54) | 22 (59.46) | 3.82 (1.32-11.07) | 1.35E-02 | 27 (38.03) | 44 (61.97) | 3.36 (1.58-7.14) | 1.65E-03 | |
| miR-17/486-5p | Low | 3 (13.04) | 20 (86.96) | 1 | 4 (16.00) | 21 (84.00) | 1 | 7 (14.58) | 41 (85.42) | 1 | |||
| High | 17 (33.33) | 34 (66.67) | 4.52 (1.02-20.16) | 4.77E-02 | 16 (34.78) | 30 (65.22) | 5.26 (1.38-20.07) | 1.51E-02 | 33 (34.02) | 64 (65.98) | 3.91 (1.5-10.18) | 5.25E-03 | |
| miR-20a/24 | Low | 10 (21.74) | 36 (78.26) | 1 | 14 (26.92) | 38 (73.08) | 1 | 24 (24.49) | 74 (75.51) | 1 | |||
| High | 8 (33.33) | 16 (66.67) | 3.03 (1.00-9.18) | 4.99E-02 | 8 (32.00) | 17 (68.00) | 3.19 (1.07-9.52) | 3.76E-02 | 16 (32.65) | 33 (67.35) | 2.37 (1.12-5) | 2.40E-02 | |
| miR-27a/146a | Low | 7 (16.67) | 35 (83.33) | 1 | 5 (15.15) | 28 (84.85) | 1 | 12 (16.00) | 63 (84.00) | 1 | |||
| High | 12 (40.00) | 18 (60.00) | 3.80 (1.23-11.72) | 2.02E-02 | 16 (37.21) | 27 (62.79) | 4.87 (1.07-22.2) | 4.10E-02 | 28 (38.36) | 45 (61.64) | 4.49 (1.87-10.75) | 7.60E-04 | |
| miR-27a/324-3p | Low | 3 (15.00) | 17 (85.00) | 1 | 4 (15.38) | 22 (84.62) | 1 | 7 (15.22) | 39 (84.78) | 1 | |||
| High | 15 (31.91) | 32 (68.09) | 6.14 (1.13-33.41) | 3.59E-02 | 16 (36.36) | 28 (63.64) | 4.48 (1.24-16.23) | 2.22E-02 | 31 (34.07) | 60 (65.93) | 3.09 (1.25-7.59) | 1.41E-02 | |
| miR-27a/331-3p | Low | 3 (12.50) | 21 (87.50) | 1 | 3 (12.50) | 21 (87.50) | 1 | 6 (12.50) | 42 (87.50) | 1 | |||
| High | 15 (32.61) | 31 (67.39) | 5.71 (1.23-26.43) | 2.58E-02 | 17 (35.42) | 31 (64.58) | 12.89 (2.35-70.74) | 3.25E-03 | 32 (34.04) | 62 (65.96) | 3.03 (1.22-7.52) | 1.67E-02 | |
| miR-27a/339-3p | Low | 6 (13.33) | 39 (86.67) | 1 | 9 (21.43) | 33 (78.57) | 1 | 15 (17.24) | 72 (82.76) | 1 | |||
| High | 9 (45.00) | 11 (55.00) | 6.17 (1.86-20.46) | 2.95E-03 | 10 (41.67) | 14 (58.33) | 7.51 (2.12-26.62) | 1.79E-03 | 19 (43.18) | 25 (56.82) | 4.07 (1.87-8.87) | 4.18E-04 | |
| miR-27a/484 | Low | 10 (19.61) | 41 (80.39) | 1 | 12 (24.00) | 38 (76.00) | 1 | 22 (21.78) | 79 (78.22) | 1 | |||
| High | 10 (41.67) | 14 (58.33) | 2.72 (1.07-6.93) | 3.63E-02 | 9 (34.62) | 17 (65.38) | 4.63 (1.48-14.51) | 8.57E-03 | 19 (38.00) | 31 (62.00) | 3.12 (1.53-6.35) | 1.69E-03 | |
| miR-25/93* | Low | 6 (20.69) | 23 (79.31) | 1 | 11 (26.19) | 31 (73.81) | 1 | 17 (23.94) | 54 (76.06) | 1 | |||
| High | 13 (32.50) | 27 (67.50) | 3.98 (1.18-13.5) | 2.64E-02 | 10 (29.41) | 24 (70.59) | 3.86 (1.28-11.59) | 1.62E-02 | 23 (31.08) | 51 (68.92) | 2.58 (1.22-5.44) | 1.31E-02 | |
| miR-30b/150 | Low | 6 (13.95) | 37 (86.05) | 1 | 11 (22.92) | 37 (77.08) | 1 | 17 (18.68) | 74 (81.32) | 1 | |||
| High | 9 (40.91) | 13 (59.09) | 8.38 (2.26-31.11) | 1.48E-03 | 8 (34.78) | 15 (65.22) | 3.32 (1.06-10.34) | 3.87E-02 | 17 (37.78) | 28 (62.22) | 4.97 (2.2-11.22) | 1.14E-04 | |
| miR-30c/423-5p | Low | 7 (16.67) | 35 (83.33) | 1 | 8 (18.18) | 36 (81.82) | 1 | 15 (17.44) | 71 (82.56) | 1 | |||
| High | 8 (36.36) | 14 (63.64) | 3.57 (1.16-11.01) | 2.69E-02 | 7 (33.33) | 14 (66.67) | 5.03 (1.28-19.68) | 2.03E-02 | 15 (34.88) | 28 (65.12) | 4.24 (1.84-9.77) | 6.75E-04 | |
| miR-106b/126 | Low | 12 (25.00) | 36 (75.00) | 1 | 9 (20.93) | 34 (79.07) | 1 | 21 (23.08) | 70 (76.92) | 1 | |||
| High | 8 (44.44) | 10 (55.56) | 4.34 (1.4-13.39) | 1.07E-02 | 10 (35.71) | 18 (64.29) | 3.46 (1.26-9.54) | 1.63E-02 | 18 (39.13) | 28 (60.87) | 3.42 (1.67-7) | 7.56E-04 | |
| miR-106b/320 | Low | 10 (23.26) | 33 (76.74) | 1 | 12 (23.53) | 39 (76.47) | 1 | 22 (23.40) | 72 (76.60) | 1 | |||
| High | 8 (34.78) | 15 (65.22) | 3.44 (1.13-10.44) | 2.95E-02 | 8 (33.33) | 16 (66.67) | 3.45 (1.21-9.82) | 2.04E-02 | 16 (34.04) | 31 (65.96) | 2.54 (1.25-5.16) | 1.01E-02 | |
| miR-142-3p/195 | Low | 11 (26.19) | 31 (73.81) | 1 | 12 (21.82) | 43 (78.18) | 1 | 23 (23.71) | 74 (76.29) | 1 | |||
| High | 9 (29.03) | 22 (70.97) | 3.98 (1.26-12.6) | 1.87E-02 | 7 (38.89) | 11 (61.11) | 4.74 (1.32-17.01) | 1.69E-02 | 16 (32.65) | 33 (67.35) | 2.10 (1.03-4.27) | 4.09E-02 | |
| miR-142-3p/342-3p | Low | 9 (18.75) | 39 (81.25) | 1 | 10 (19.61) | 41 (80.39) | 1 | 19 (19.19) | 80 (80.81) | 1 | |||
| High | 11 (37.93) | 18 (62.07) | 3.67 (1.15-11.7) | 2.80E-02 | 9 (42.86) | 12 (57.14) | 4.98 (1.52-16.27) | 7.91E-03 | 20 (40.00) | 30 (60.00) | 3.81 (1.84-7.9) | 3.26E-04 | |
| miR-186/342-3p | Low | 7 (14.58) | 41 (85.42) | 1 | 15 (27.27) | 40 (72.73) | 1 | 22 (21.36) | 81 (78.64) | 1 | |||
| High | 11 (39.29) | 17 (60.71) | 7.75 (2.38-25.22) | 6.78E-04 | 9 (36.00) | 16 (64.00) | 3.02 (1.06-8.67) | 3.93E-02 | 20 (37.74) | 33 (62.26) | 3.61 (1.77-7.37) | 4.32E-04 | |
| miR-191/331-3p | Low | 3 (15.79) | 16 (84.21) | 1 | 5 (20.00) | 20 (80.00) | 1 | 8 (18.18) | 36 (81.82) | 1 | |||
| High | 13 (29.55) | 31 (70.45) | 12.95 (1.63-103.05) | 1.55E-02 | 15 (32.61) | 31 (67.39) | 7.59 (1.48-38.88) | 1.50E-02 | 28 (31.11) | 62 (68.89) | 2.62 (1.07-6.42) | 3.44E-02 | |
| miR-151-3p/423-5p | Low | 12 (28.57) | 30 (71.43) | 1 | 12 (25.00) | 36 (75.00) | 1 | 24 (26.67) | 66 (73.33) | 1 | |||
| High | 3 (14.29) | 18 (85.71) | 0.07 (0.01-0.57) | 1.23E-02 | 5 (21.74) | 18 (78.26) | 0.16 (0.04-0.74) | 1.85E-02 | 8 (18.18) | 36 (81.82) | 0.40 (0.17-0.96) | 4.06E-02 | |
| miR-223*/342-3p | Low | 7 (19.44) | 29 (80.56) | 1 | 9 (24.32) | 28 (75.68) | 1 | 16 (21.92) | 57 (78.08) | 1 | |||
| High | 13 (37.14) | 22 (62.86) | 4.71 (1.47-15.09) | 8.98E-03 | 13 (32.50) | 27 (67.50) | 4.20 (1.47-11.98) | 7.29E-03 | 26 (34.67) | 49 (65.33) | 2.39 (1.23-4.65) | l.00E-02 | |
HR, hazard ratio; CI, confidence interval.
a
Adjusted for age, sex, smoking history, clinical stage, and treatment regimen.