. Author manuscript; available in PMC: 2019 Apr 1.

Published in final edited form as: Neurobiol Learn Mem. 2018 Feb 13;150:1–12. doi: 10.1016/j.nlm.2018.02.008

Table 1.

Statistical results (F and p values) and group numbers (n) for Experiment 1. Factorial ANOVA and Newman-Keuls post-hoc tests were used to evaluate changes in gene expression as a function of age at preexposure (using the single-exposure protocol). Gene expression was analyzed in PD17 and PD24 animals following the preexposure (Experiment 1A) and training (Experiment 1B) and in PD31 animals following training (Experiment 1C).

Experiment 1.A: Single-Exposure CPFE Preexposure day Egr-1 expression PD17 and PD24

	AC			PL			IL
	n (HC, Alt-Pre, Pre)			n (HC, Pre, Alt-Pre)			n (HC, Pre, Alt-Pre)

PD17	13, 11, 12			13, 11, 11			13, 12, 12
PD24	12, 10^**, 11			12, 10^*, 13^			12, 10^*, 12^
	F	p		F	p		F	p

Age	14.36	<. .001	PD24>PD17	15.63	<.001	PD24 >PD17	14.63	<.001	PD24 > PD17

Preexposure	4.15	0.02	Alt-Pre > HC	4.92	0.01	Pre, Alt-Pre > HC	16.66	<.001	Pre, Alt-Pre > HC

Age x Preexposure	3.88	0.03	PD 24 Pre, Alt-Pre > PD 17, HC	4.18	0.02	PD 24 Pre, Alt-Pre > PD 17, HC	4.04	0.02	PD 24 Pre, Alt-Pre > PD 17, HC

	OFC			dHPC			dLA
	n (HC, Pre, Alt-Pre)			n (HC, Pre, Alt-Pre)			n (HC, Alt-Pre, Pre)

PD17	13, 12, 11			11, 12, 12			12, 12, 12
PD24	11, 11^*, 12^			12, 10, 11^**			12, 11^*, 10^*
	F	p		F	p		F	p

Age	15.57	<.001	PD24>PD17	8.04	<01	PD24>PD17	20.6	<.001	PD24>PD17

Preexposure	8.74	.<001	Pre, Alt-Pre> HC	4.84	0.01	Pre, Alt-Pre > HC	8.27	<.001	Pre, Alt-Pre > HC

Age x Preexposure	4.89	0.01	PD24 Pre, Alt-Pre > PD 17, HC	3.14	0.0502	NS	6.03	<.01	PD 21 Pre, Alt-Pre > PD 17, HC

Experiment 1B: Single-Exposure CPFE Training day Egr-1 expression PD17 and PD24

	AC			PL			IL
	n (HC, Alt-Pre, Pre)			n (HC, Pre, Alt-Pre)			n (HC, Pre, Alt-Pre)

PDI7	13, 12^, 11^			13, 12^*, 11^*			13, 12^*, 11^
PD24	12, 12^, 10^			12, 10^*, 11^			12, 10^*, 11^*
	F	p		F	p		F	p

Age	0.18	0.68	NS	0.55	0.46	NS	9.39	<.01	PD24 > PD27

Preexposure	6.24	<.01	Pre, Alt-Pre > HC	20.94	<.001	Pre. Alt-Pre > HC	16.12	<.001	Pre, Alt-Pre > HC

Age x Preexposure	0.23	0.8	NS	1.54	0.22	NS	2.46	0.09	NS

	OFC			dHPC			dLA
	n (HC, Pre, Alt-Pre)			n (HC, Pre, Alt-Pre)			n (HC Alt-Pre, Pre)

PD17	13, 13^*, 11^			12, 12^**, 9^			12, 10^*, 11^*
PD24	12, 10^*, 11^			12, 10^**, 11^			12, 11^**, 12
	F	P		F	p		F	p

Age	0.47	0.49	NS	1.54	0.22	NS	0.99	0.32	NS

Preexposure	11.50	<.001	Pre, Alt-Pre > HC	15.72	<.001	Alt-Pre > Pre > HC	13.37	<.001	Alt-Pre > Pre > HC

Age x Preexposure	0.15	0.86	NS	0.45	0.64	NS	1.52	0.23	NS

Experiment 1C: Single-Exposure CPFE Training day Egr-1 expression PD31

	AC			PL			IL
	n (HC, Alt-Pre, Pre)			n (HC, Pre, Alt-Pre)			n (HC, Pre, Alt-Pre)

PD31	12, 12^, 11^			12, 12^, 12^**			12, 12^, 12^*
	F	p		F	p		F	p

Preexposure	7.08	< .01	Pre, Alt-Pre > HC	12.02	<.001	Pre > Alt-Pre >HC	13.32	< .001	Pre > Alt-Pre >HC

	OFC			dHPC			dLA
	n (HC, Alt-Pre, Pre)			n (HC, Pre, Alt-Pre)			n (HC, Pre, Alt-Pre)

PD31	12, 12^, 12^			11, 12, 12			11, 12, 12
	F	p		F	p		F	p

Preexposure	3.80	0.03	Pre, Alt-Pre > HC	2.87	0.069		3.04	0.059

Asterisks (next to group n) represent Newman-Keuls post hoc tests comparing experimental groups to homecage (HC) controls.

p < .05,

^**

p < .01,

^***

p < .001.

Main or interaction effects are described by inequality signs (> or <) and non-significant effects are listed as “NS.”