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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 12;200(8):2786–2797. doi: 10.4049/jimmunol.1701668

FIGURE 11.

FIGURE 11

Kinetics and dose response curve for human Factor D in the rabbit RBC hemolysis and LPS microplate assays for AP. A-D were performed in FD-depleted human serum reconstituted with hFD. A) Dose response curve for hFD with a 5 min incubation. B) Kinetics of hemolysis when using 400 ng/ml hFD. C) Dose response curve of hFD when used with a 30 min incubation. RBC hemolysis data are expressed as percent hemolysis. D) Dose response curve for hFD added to FD-depleted human serum in the LPS microplate assay. E) Dose response curve for FD mixing experiment using WT and FD−/− mouse serum in the LPS microplate assay.