Table I.
Details of 49 proteins selected for in silico analysis of epitope binding affinity predictions using NetMHCIIpan2.1
| Protein name | Product | GeneDB/GenBank Accession | Reference |
|---|---|---|---|
| A2 LINJ220670 | hypothetical protein | UniProtKB - A4HZU7_LEIIN | (34) |
| CPL2 | cathepsin L-like protease | LdBPK_080960.1 | (35) |
| CPA/CBA | cysteine peptidase A | LdBPK_191460.1 | (36) |
| CPB | cysteine peptidase B | LdBPK_070600.1 | (36) |
| CPC | cysteine peptidase C | LdBPK_290860.1 | (37) |
| gp46/PSA/M-2 | surface membrane protein gp46 | LdBPK_311490.1 | (38) |
| gp63/MSP | GP63, leishmanolysin | GenBank: ACT31401.1 | (39) |
| HASPB1/K26 | hydrophilic acylated surface protein B | GenBank: CAA09789.1 | (40) |
| HbR | hemoglobin receptor | LdBPK_210300.1; | (41) |
| Hsp70 | HSP70-like protein | LdBPK_283000.1 | (39) |
| J41 | hypothetical protein | LdBPK_331620.1 | (42) |
| J89 | hypothetical protein | LdBPK_260990.1 | (42) |
| KMP11 | kinetoplastid membrane prot-1 | LdBPK_352260.1 | (43) |
| L15 | anaphase promoting complex subunit | LdBPK_354570.1 | (42) |
| L21 | nucleolar protein family a member | LdBPK_343810.1 | (42) |
| lmd29 | hypothetical protein | LdBPK_180600.1 | (42) |
| L302.06 | hypothetical protein, conserved | LdBPK_040220.1 | (42) |
| L31/584C | 60S ribosomal subunit L31 | LdBPK_353330.1 | (42) |
| L37 | 60S ribosomal L37 | LdBPK_342620.1 | (42) |
| L5 | 60S ribosomal L5 | LdBPK_351870.1 | (44) |
| L3 | ribosomal protein L3-like | LdBPK_323320.1 | (44) |
| LACK/p36 | activated protein kinase c receptor | LdBPK_282970.1 | (45) |
| LeIF | eukaryotic initiation factor 4a | LdBPK_010790.1 | (46) |
| Lepp12 | phosphoprotein lepp12 | LdBPK_365970.1 | (47) |
| LmSTI1 | stress-induced protein sti1 | LdBPK_081020.1 | (48) |
| LPG3 | lipophosphoglycan biosynthetic | LdBPK_290790.1 | (49) |
| M18 | amastin-like protein | LdBPK_080720.1 | (42) |
| N52 | vacuolar ATP synthase subunit | LdBPK_120480.1 | (42) |
| NH36 | nonspecific nucleoside hydrolase | LdBPK_181570.1 | (50) |
| PABP1 | polyadenylate-binding protein 1 | LdBPK_355360.1 | (51) |
| PABP2 | polyadenylate-binding protein 2 | LdBPK_354200.1 | (51) |
| PABP3 | poly(A)-binding protein, putative | LdBPK_250080.1 | (51) |
| Pxn4 | peroxidoxin_4 | LdBPK_230050.1 | (52) |
| PO | 60S acidic ribosomal protein P0 | LdBPK_072480.1 | (53) |
| P25 | hypothetical protein | LdBPK_354800.1 | (42) |
| P31 | hypothetical protein, conserved | LdBPK_342300.1 | (42) |
| PRP-2 | paraflagellar rod protein 2C | LdBPK_161510.1 | (54) |
| Q24 | dynein light chain, flagellar outer arm | LdBPK_320240.1 | (42) |
| Q51 | hypothetical protein | LdBPK_301750.1 | (42) |
| R32 | hypothetical protein | LdBPK_333110.1 | (42) |
| R71 | 60S ribosomal protein L22 | LdBPK_363430.1 | (42) |
| S4 | 40S ribosomal protein S4 | LdBPK_131120.1 | (55) |
| S33 | eukaryotic initiation factor 5a | LdBPK_250740.1 | (42) |
| SOD B1 | iron superoxide dismutase | LdBPK_321910.1 | (56) |
| SMT | sterol 24-c-methyltransferase | LdBPK_362520.1 | (57) |
| TSA/TRYP | tryparedoxin peroxidase | LdBPK_151140.1 | (31) |
| TUZIN1 | Tuzin, putative (62.3 kDa) | LdBPK_342640.1 | (58) |
| TUZIN2 | Tuzin, putative (58.7 kDa) | LdBPK_080730.1 | (58) |
| TUZIN3 | Tuzin, putative (70.2 kDa) | LdBPK_080740.1 | (58) |
Except for A2, all protein sequences were based on L. donovani sequences, as represented in GeneDB or GenBank. References to vaccine candidacy are not comprehensive, especially where multiple studies have been undertaken. Where possible we quote a reference where pre-clinical vaccine studies were undertaken in a model of visceral leishmaniasis.