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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Hepatology. 2018 Apr 19;67(6):2320–2337. doi: 10.1002/hep.29585

Figure 6. β- and γ-catenin loss in hepatocytes leads to deregulation of several key junctional molecules.

Figure 6

  1. Representative WB shows decreased E-cadherin at 10d-14d, and occludin, claudin-3 and -5 at 12d-14d in DKO2 as compared to 14d dWT. Claudin-2 was absent from 7d in DKO2 livers. GAPDH shows protein loading.
  2. RT-PCR shows significant decrease in expression of claudin-2 (p<0.001) in DKO2 at 7d onwards as compared to dWT livers. Significant decrease in occludin and E-cadherin mRNA in DKO2 livers was evident only at 14d (p<0.001).
  3. Membrane localization of E-cadherin evident in dWT livers was visibly reduced in DKO2 at 12d and appeared as punctate cytoplasmic staining by confocal microscopy.
  4. Confocal microscopy showed membrane localization of claudin-3 and -5 in dWT livers, which was reduced in DKO2 at 12d.
  5. TIRF imaging revealed membrane localization of E-cadherin in dWT liver sections, which was absent in DKO2 livers at 12d.
  6. TIRF imaging revealed membrane localization of occludin in dWT liver sections, which was absent in DKO2 livers at 12d. G. Scale bars: 50μm.