Figure 5. αE-catenin-E-cadherin complex is a direct target of miR-720 and miR-720 silencing attenuates EMT and metastasis associated genes.
A–B) Transient transfection of anti720 caused a significant increase in αE-catenin and E-cadherin protein levels compared to control in both cell lines. C) Complimentary binding sites for miR-720 in 3′UTR of αE-catenin and E-cadherin. D–E) Luciferase assays showing decreased reporter activity after co-transfection of either wild type 3′UTR sequences compared to non-specific control 3′UTR with miR-720 in 786-O and A498 cells. F–I) Decreased Akt, CTNNB1 (β-catenin) and CD44 expression observed at mRNA (F–G) and protein (H–I) levels after miR-720 silencing in both A498 and 786-O RCC cell lines.