Fig. 5. Insertion sites of csep1 genes in C. concisus genomes.

a The flanking genes of the csep1^P gene in the pICON plasmid of strain P2CDO4, P20CDO-S2 and P20CDO-S3 were almost identical, indicating the csep1^P was inserted at the same location in the pICON plasmid of different strains. b The flanking genes of the csep1^C gene in all the csep1^C-positive strains shared similar patterns, strains P2CDO4 and H11O-S2 are shown here as examples. The two genes (boxed) immediately upstream of the csep1^C gene were absent in all csep1^P-negative strains, while other flanking genes were present, although distantly located, strain P16UCO-S1 is shown here as an example. c The csep1^C2 gene was inserted at a rare spot within the chromosome, in which only appeared in strain P11CDO-S1. In the remaining strains with similar flanking gene arrangements, there was either no gene insertion, such as strain P2CDO3; or insertion of other genes such as strain P16UCO-S2. Nucleotide sequences sharing more than 80% identity were shaded in grey. Nucleotide positions referred to the positions within the contig, except strain P2CDO4 which had the genome sequenced without gap. Gene sizes in strains between a, b and c were not on scale. HSDH homoserine dehydrogenase, PDA polysaccharide deacetylase