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. 2018 Apr 10;9:1376. doi: 10.1038/s41467-018-03832-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — NLP and NIGT1 activity affects tZ-type cytokinin content. Cytokinin contents in the seedlings treated with 1 mM KNO₃ or KCl for 24 h were measured. The cytokinin contents in two NLP6-SUPRD lines were compared with those in its parental line (4xNRE-35Smin-GUS)²¹, and the cytokinin contents in the nlp6 nlp7-1 mutant, the nigt1 quadruple mutant (nigtQ-3), and the complementation line of nigtQ-3, which was produced by expression of NIGT1.2, were compared with those in Col seedlings. The cytokinin contents in the NIGT1 overexpressor (NIGT1.2-GFP-OX), which was produced by the overexpression of NIGT1.2 fused to GFP, were compared with those in the GFP overexpressor line (GFP-OX). Values are mean ± s.d. (n = 4~5). Different letters denote significant difference by Tukey’s HSD (p < 0.05)