Figure 5.

Male offspring from metformin exposed dams have few changes in expression or protein levels of candidates in the granule exocytosis pathway. (a) Expression of candidate granule exocytosis machinery from islets isolated from 4–5 month old animals, n = 9. (b) A representative western blot of CSPα and quantification of CSPα corrected to β-actin from male islets. (c) A representative western blot and quantification for SNAP25. The representative western blot images are selected from 2 adjacent wells of a larger western blot shown in Supplemental Fig. 2. These experiments represent n = 9 animals run in 3 separate experiments.