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. 2018 Apr 10;8:5776. doi: 10.1038/s41598-018-23822-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription of major satellites (A) Relative expression (CNRQ) of major satellite transcripts by qRT-PCR analysis normalized to Sdha and Pbgd housekeeping genes. Each point is an independent biological replicate. Statistically significant data are indicated by an asterisk (P < 5%, Mann-Whitney U test). (B) Detection of major satellite transcripts using RNA-FISH. Transcripts localization appears as green foci while DNA is counterstained with DAPI (red). Percentage of positive nuclei is indicated for each cell type as well as an enlarged representative nucleus (indicated in the cell field by a green arrowhead). Scale bars represent 5 µm. (C) Boxplots showing the number of foci/ positive nucleus. Mean is indicated by a cross. Statistically significant data are indicated by an asterisk (P < 0.3%, Student test).