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. 2018 Apr 10;8:5723. doi: 10.1038/s41598-018-24152-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Pneumococci-induced KLF4 expression is partly mediated by TLR9, MyD88 and TRIF. MyD88- (a), TRIF- (c), TLR2,3,4,7,9- (e) and TLR9- (g) knockout BMMs were stimulated with 10⁶ CFU/ml R6x and 100 ng/ml LPS (a,c) or 1 µg/ml CpG (g). Cell lysates were collected after 6 h and analysed for KLF4 expression and actin for equal protein load. All blots show one representative blot out of 3 independent experiments with similar results. The densitometry of the KLF4 and actin bands of the blots was quantified using Odyssey 2.0 infrared imaging system. The ratio between the KLF4 and actin densitometry was calculated. (b) shows the quantification of the blots of experiment (a), (d) for (c), (f) for (e) and (h) for (g). Data represents mean of 3 independent experiments. Differences were indicated as follows: *p < 0.05; ***p < 0.001.