Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 10;8:5723. doi: 10.1038/s41598-018-24152-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Pneumococci-dependent KLF4 induction in BMMs is not mediated by type I IFN signaling. WT or IFNAR-deficient (IFNAR KO) BMMs were stimulated with 10⁶ CFU/ml R6x (a) and wt BMMs were exposed to 500 U/ml IFNβ alone or in combination with 10⁶ CFU/ml R6xΔlytA (c). Cell lysates were collected 6 h after stimulation and analysed for KLF4 expression. Actin was used to confirm equal protein load. Both blots show one representative result out of 2 independent experiments with similar results. The densitometry of the KLF4 and actin bands of the blots was quantified using Odyssey 2.0 infrared imaging system. The ratio between the KLF4 and actin densitometry was calculated. (b) shows the quantification of the blots of experiment (a) and (d) for (c). Data represents mean of 2 independent experiments.