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. 2018 Apr 10;8:5781. doi: 10.1038/s41598-018-23728-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE and western blot analysis of PvAARP and PkAARP. (a) Crude recombinant proteins of full-length PvAARP and PkAARP [rPvAARP-FL and rPkAARP-FL] were expressed with the WGCF expression system. (b) Recombinant PvAARP-N [Pv, 0.5 µg] and PkAARP-N [Pk, 0.5 µg] proteins were expressed in E. coli and purified to a single band [arrowhead]. (c) Specific band have been detected with anti-glutathione S-transferase antibody [Gst], mouse serum [M] and rabbit immunized [R] with PvAARP-N or PkAARP-N. (d) P. knowlesi A1-H.1 parasite lysate was recognized with anti-PvAARP-N [α-Pv] and anti-PkAARP-N [α-Pk] antibody, nor non-immunized rabbit (α-NI). The full length of SDS-PAGE and western blots are presented in the Supplementary Fig. S2. (e) Immunofluorescence assay of anti-PvAARP-N and PkAARP-N with P. knowlesi A1-H.1. pRBC, parasitized-red blood cells; uRBC, uninfected-red blood cells; DAPI, 4’,6-diaminidino-2-phenylindole. Bars indicate 5 μm.