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Use of RosetteSep™ on total blood samples prior to Polymophprep™ protocol allows to obtain a higher monocytic population purity. Peripheral blood mononuclear cells were analyzed by flow cytometry. CD3+ (lymphocytes, green dots), CD14+ (monocytes, red dots), and CD66+ (granulocytes, blue dots) cell contents were monitored. Representative dot plots (A,B) and cell population percentage (C) are shown. & p < 0.05 vs. RosetteSep™ + Polymorphprep™.
