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. 2018 Apr 4;9:632. doi: 10.3389/fimmu.2018.00632

Figure 5.

Figure 5

Kinetic of monocytic-precursors fusion toward osteoclastic phenotype and osteoclast-resorbing activity on dentin disks. Phalloidin-AlexaFluor®488/DAPI stainings on freshly isolated human primary peripheral blood mononuclear cells (Polymorphprep™ protocol) are shown for 7 (A), 14 (B), and 21 (C) days culture periods. Some osteoclasts are highlighted with white arrowheads. Scale bar = 200 µm. Cells plated on dentin disk for 7 (D) and 14 (E) were observed by SEM. Pits (white arrowheads) and trenches (yellow arrowheads) are visible. Scale bar = 100 µm.