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. 2018 Apr 4;9:613. doi: 10.3389/fmicb.2018.00613

FIGURE 1.

FIGURE 1

Identification of BrYV systemic infection defective mutant in Nicotiana benthamiana. (A) Symptoms of BrYV and the mutant (BrYVm) in N. benthamiana agroinfiltrated leaves 5 dpi. (B) Western blot analysis of the accumulation of BrYV CP, MP, and CP-RTD in BrYV and BrYVm agroinfiltrated N. benthamiana leaves at 2 dpi. Coomassie brilliant blue (Coom.) staining is shown as a loading control. Northern blot analysis of RNAs extracted from agroinfiltrated N. benthamiana leaves at 2 dpi (C) and upper leaves at 14 dpi (D). rRNA, loading control of methylene blue stained ribosomal RNA.