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. 2018 Apr 4;9:613. doi: 10.3389/fmicb.2018.00613

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Copyright © 2018 Zhang, Zhao, Li, Xiang, Dong, Zhang, Wang, Li, Yu and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Systemic infection after leaves co-infiltration of BrYV P3a mutants and Pea enation mosaic virus 2 (PEMV 2). (A) Northern blotting of RNAs extracted from different treated N. benthamiana upper leaves 14 dpi. rRNA, loading control of methylene blue stained ribosomal RNA. (B) Western blotting analyses of the accumulation of BrYV CP and PEMV 2 MP extracted from N. benthamiana upper leaves 14 dpi using specific antisera raised against BrYV CP and against PEMV 2 MP. Coomassie brilliant blue (Coom.) staining is shown as a loading control. (C) The symptoms of N. benthamiana upper leaves by co-infiltration with full-length cDNA clones of PEMV 2 and BrYV or its mutants.