Table 1.

Primer sequences used in this study.

Primer name	Sequence (5′–3′)	Restriction sites
ZmDREB4.1-F	ATGGAGCACGAGCAGGTGTCG
ZmDREB4.1-R	TCAATGTTCCCAAAGCAGCAAGG
ZmDREB4.1-ORF-F	TCTAGAATGGAGCACGAGCAGGTGTCG	Xba I
ZmDREB4.1-ORF-R	GAGCTCTCAATGTTCCCAAAGCAGCAAGG	Sac I
ZmDREB4.1-qRT-F	CATTGGAGTTGGACTGGGCTATACG
ZmDREB4.1-qRT-R	ATGGTCAATGTTCCCAAAGCAGCAA
ZmDREB4.1-SL-F	TCTAGAATGGAGCACGAGCAGGTGTCG	Xba I
ZmDREB4.1-SL-R	CCCGGGATGTTCCCAAAGCAGCAAGGGCT	Sma I
ZmDREB4.1-TA-F	GAATTCATGGAGCACGAGCAGGTGTCG	EcoR I
ZmDREB4.1-TA-R	GTCGACTCAATGTTCCCAAAGCAGCAAGGG	Sal I
ZmDREB4.1-YH-F	GAATTCATGGAGCACGAGCAGGTGTCG	EcoR I
ZmDREB4.1-YH-R	GGATCCTCAATGTTCCCAAAGCAGCAAGG	BamH I
ZmDREB4.1-PE-F	GAATTCCTCAACGGCTCGTCGTCGCC	EcoR I
ZmDREB4.1-PE-R	GTCGACTCAAGCCGGCACAGTGGCCGC	Sal I
mini35S-F	AAGCTTATCTCCACTGACGTAAGGGATG	Hind III
mini35S-R	CCATGGCGTGTTCTCTCCAAATGAAATG	Nco I
DRE element	GATATACTACCGACATGAGTTC
mDRE element	GATATACTAAAAAAATGAGTTC
IPT3-qRT-F	CCAAGAACTTCTGTGACATGGCTTCA
IPT3-qRT-R	ACCTCGTTGACAAGTCCTTTCTCGA
IPT5-qRT-F	AAGCAGAGGTTGTGAACAGTGACAA
IPT5-qRT-R	TCGACCCTCCAGCAATTATCGGTAG
YUCCA9-qRT-F	TTATTGTAGGTGCAGGTCCATCGGG
YUCCA9-qRT-R	TGTCTCTTTGTTGGGTACTCAGGGT
YUCCA10-qRT-F	TTCGATGCCCAAAACATAGCCACAG
YUCCA10-qRT-R	AATCACATCCGATCGACCCCTTCAT
YUCCA11-qRT-F	AGGATTAGATGGCTTTGGTGGGACA
YUCCA11-qRT-R	ACAGGGCTACGAACAATAATGGAGGT
GA2ox2-qRT-F	AGCAGATTGGATGTAATGGCGATTCC
GA2ox2-qRT-R	TCTCACACGCCATTTTCTTCACTGC
GA2ox3-qRT-F	TCAGCAATGAAGAAAATGGCATGTGA
GA2ox3-qRT-R	GCACCAAATCCAATCAAATTTCTGCCA
GA2ox4-qRT-F	AAAGTCGTCAACCATAGCGTCCCTA
GA2ox4-qRT-R	TTCGACCCAACCCAAATCACCATTG
GA3ox2-qRT-F	GATGGTATTGCTGGTTATGGCGTGG
GA3ox2-qRT-R	CCACATCAGTCTTCCGGCTAGTTTT
GA20ox1-qRT-F	GCGTAGGGAGGGAACACTTCAAAGA
GA20ox1-qRT-R	AAACTTGAAGCCCGCCAACACTATC
GA20ox2-qRT-F	AGGCAATGAAGAGGGTAGGACTTGT
GA20ox2-qRT-R	GTGCAGTTCCAAGGGCTAAATCAGG
GA20ox3-qRT-F	CCCTTGAGGCTTGGACAAATGGAAG
GA20ox3-qRT-R	AGATGCCTCAGTTCAGCCCATGTAA
ZmActin-F	GTTGGGCGTCCTCGTCA
ZmActin-R	TGGGTCATCTTCTCCCTGTT
NtActin-F	GAGCGGGAAATTGTCAGGGA
NtActin-R	GAAACGCTCAGCACCAATGG