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. 2018 Apr 4;9:660. doi: 10.3389/fimmu.2018.00660

Figure 1.

Figure 1

Reduction of KLRG1+ CD8 EM T cells in G-protein-coupled receptor 18 (GPR18)-deficient peripheral blood lymphocytes (PBL). (A) Gpr18 transcript abundance in the indicated cell subsets relative to Hprt. Each point indicates cells sorted from an individual mouse and lines indicate mean ± SEM. n = 3 or 4 in each populations. EM, effector-memory; CM, central memory; IEL, intraepithelial lymphocytes. (B) Flow cytometric analysis of CD44 and CD62L expression in CD8+ TCRβ+ PBL from the indicated mature (6 months old) mice. Numbers show percentage of cells in the indicated gate. (C) Frequency of EM (CD44hi CD62Llo), CM (CD44hi CD62Lhi), and naive (CD44lo CD62Lhi) CD8+ or CD4+ TCRβ+ cells in PBL from young (2 months old, left panel) or mature (6 months old, right panel) Gpr18+/−and Gpr18−/− mice. Left panel: Gpr18+/−, n = 8; Gpr18−/−, n = 8. Right panel: Gpr18+/−, n = 21; Gpr18−/−, n = 16. (D) Flow cytometric analysis of KLRG1 expression in CD8 EM PBL from the indicated mature (6 months old) mice. Numbers show percentage of cells in the indicated gate. (E) Frequency of KLRG1+ CD8 EM PBL in indicated young (2 months old) or mature (6 months old) mice. Young adults: Gpr18+/−, n = 8; Gpr18−/−, n = 8. Mature adults: Gpr18+/−, n = 21; Gpr18−/−, n = 16. Each point represents data from an individual mouse and lines represent means ± SEM (C,E). ***p < 0.001, **p < 0.01, *p < 0.05, “n.s.” p > 0.05 by Student’s t-test (C,E).