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. 2018 Apr 4;9:660. doi: 10.3389/fimmu.2018.00660

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Copyright © 2018 Sumida and Cyster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduction of KLRG1⁺ CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44^hi CD62L^lo), central memory (CM) (CD44^hi CD62L^hi), and naive (CD44^lo CD62L^hi) populations in CD8⁺ or CD4⁺ TCRβ⁺ splenocytes in mature mice (6 months old) of the indicated type. Gpr18^+/⁻, n = 11; Gpr18^−/−, n = 12. (B) Number of CD8⁺ TCR β⁺ splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45⁺TCRβ⁺ cells. Gpr18^+/⁻, n = 13; Gpr18^−/−, n = 14. (C) Frequency of KLRG1⁺ cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18^+/⁻, n = 9; Gpr18^−/−, n = 8. (D) Numbers of KLRG1⁺ CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18^+/⁻, n = 8; Gpr18^−/−, n = 8. (E) Frequency of EM, CM, and naive populations in CD8⁺ or CD4⁺ TCRβ⁺ lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18^+/⁻, n = 7; Gpr18^−/−, n = 6. (F) Frequency of KLRG1⁺ populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18^+/⁻, n = 8; Gpr18^−/−, n = 7. (G) Percentages of Gpr18^−/− or control Gpr18^−/− EM cells in gp33 tetramer⁺ CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1^lo CD127^lo), memory precursor effector cells (MPEC) (KLRG1^lo CD127^hi), and short-lived effector cells (SLEC) (KLRG1^hi CD127^lo) in gp33 tetramer⁺ CD44^hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18^+/⁻, n = 4; Gpr18^−/−, n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H). ***p < 0.001, **p < 0.01, *p < 0.05, “n.s.” p > 0.05 by Student’s t-test.